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epcam magnetic separation 4634

epcam magnetic separation 4634

Using the immunoaffinity based Dynabeads magnetic separation technology these subsets can be selectively isolated and studied The cell culture media must first be enriched for exosomes This can be done quickly and efficiently using Total Exosome Isolation Reagent from cell culture media or traditional ultracentrifugation

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Photoresponsive immunomagnetic nanocarrier for capture
Photoresponsive immunomagnetic nanocarrier for capture

After incubation with antiEpCAMIgGBPMSAMBs for 30 min and magnetic scaffold separation in the dark few cells remained on the 96well cellculture plate Fig S6b†

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Membrane Hsp70A Novel Target for the Isolation of
Membrane Hsp70A Novel Target for the Isolation of

The CellSearch system the FDAapproved “gold standard”combines a magnetic separation technique based on EpCAM antibodycoated particles with subsequent cytokeratin CK staining and a microscopic analysis of the isolated cells

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Circulating Tumor Cell Detection In Epithelial Ovarian
Circulating Tumor Cell Detection In Epithelial Ovarian

After magnetic separation the captured cells were fixed with 4 paraformaldehyde 10 mins permeabilized with 01 Triton X100 10 mins blocked with 2 BSA 30 mins and incubated with Alexa Fluor 568labeled anticytokeratin 19 CK19 monoclonal antibody Alexa Fluor 488labeled antiCD45 monoclonal antibody and DAPI for 30 mins

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EpCAM D4K8R XP Rabbit mAb Cell Signaling Technology
EpCAM D4K8R XP Rabbit mAb Cell Signaling Technology

IMPORTANT Prewash 73778 magnetic beads just prior to use Transfer 20 μl of bead slurry to a clean tube Place the tube in a magnetic separation rack for 1015 seconds Carefully remove the buffer once the solution is clear Add 500 μl of 1X cell lysis buffer to the magnetic bead pellet briefly vortex to

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Abstract Materials and Methods
Abstract Materials and Methods

magnetic nanoparticles for columnfree magnetic selection Immunomagnetic separation of EpCAM cultured human mammary epithelial cells using a TAC incorporating the antiEpCAM antibody clone VU1D9 resulted in purities of 93±1 and recoveries of 47±5 mean ± SEM n7 Immunomagnetic separation of CD49fexpressing human mammary cells was

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Circulating tumor cell isolation culture and downstream
Circulating tumor cell isolation culture and downstream

Jul 01 2018 · Antibody based CTC separation pluriSelect uses pluriBead carrying a tumorassociated antiEpCAM antibody Nonmagnetic cell separation can be added directly to a whole blood sample Currently limited for use in colon carcinoma diagnostics Reactive Ion Etching RIE Chen et al 2012a Nalepa et al 2013

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CST EpCAM D1B3 Rabbit mAb
CST EpCAM D1B3 Rabbit mAb

IMPORTANT Prewash 73778 magnetic beads just prior to use Transfer 20 μl of bead slurry to a clean tube Place the tube in a magnetic separation rack for 1015 seconds Carefully remove the buffer once the solution is clear Add 500 μl of 1X cell lysis buffer to the magnetic bead pellet briefly vortex to

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CD326 EpCAM MicroBeads mouse
CD326 EpCAM MicroBeads mouse

Proceed to magnetic separation 23 23 Magnetic separation Choose an appropriate MACS Column and MACS Separator according to the number of total cells and the number of CD326 EpCAM cells For details refer to the table in section 14

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Emerging Technologies for CTC Detection Based on
Emerging Technologies for CTC Detection Based on

5 Magnetic Depletion Technologies The enrichment for targeted cells by depletion of the unwanted cells is a general strategy beyond the search for CTCs and specialized magnetic separation instrumentation and reagents are available commercially 31 32We have recently developed a negative CTC enrichment strategy that relies on a combination of viscous flow that facilitates recovery of the

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Protocol MojoSort Mouse CD326 EpCAM Positive
Protocol MojoSort Mouse CD326 EpCAM Positive

Application notes To use this product in magnetic separation columns a titration of the Nanobeads should be performed Optimal concentration for magnetic separation columns is lotspecific Please contact BioLegend Technical Service tech for further assistance on how to use MojoSort Nanobeads in magnetic separation columns

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EpCAM E6V8Y XP Rabbit mAb Mouse Preferred Cell
EpCAM E6V8Y XP Rabbit mAb Mouse Preferred Cell

IMPORTANT Prewash 73778 magnetic beads just prior to use Transfer 20 μl of bead slurry to a clean tube Place the tube in a magnetic separation rack for 1015 seconds Carefully remove the buffer once the solution is clear Add 500 μl of 1X cell lysis buffer to the magnetic bead pellet briefly vortex to

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Photoresponsive immunomagnetic nanocarrier for
Photoresponsive immunomagnetic nanocarrier for

magnetic separation 7Aminocoumarin was synthesized and reacted with biotin to form a photoresponsive linker Scheme 1a This photoresponsive linker was then used to bridge the capture antibody and streptavidin SA modi ed MBs magnetic hysteresis loop and timedependent magnetic separation efficiency are shown in Fig S1† Scheme 1b

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MagneticActivated Cell Sorting an overview
MagneticActivated Cell Sorting an overview

Separation can be achieved by first coating the magnetic beads with an antibody which is known to selectively bind to the desired cell type and incubating them with the sample 45 Once the cells have bound to the particles the mixture is passed through a small column under the influence of a strong magnetic

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Photoresponsive immunomagnetic nanocarrier for capture
Photoresponsive immunomagnetic nanocarrier for capture

After incubation with antiEpCAMIgGBPMSAMBs for 30 min and magnetic scaffold separation in the dark few cells remained on the 96well cellculture plate Fig S6b†

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Magnetic separation of organelles using magnetic beads
Magnetic separation of organelles using magnetic beads

After separation of the A33exsosomes the residual CCM was treated with antiEpCAMcoupled SPIONbased magnetic beads EpCAM MicroBeads Miltenyi Biotec to isolate EpCAMexosomes Two common exosome markers Alix and TSG101 were detected in both A33exosomes and EpCAMexosomes by WB

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EGFRBased Immunoisolation as a Recovery Target for Low
EGFRBased Immunoisolation as a Recovery Target for Low

Oct 06 2016 · We next optimised the appropriate concentration of beads for magnetic separation by testing a concentration range of antiEpCAM magnetic beads in SW480 colon tumour cells For this increasing numbers of cells were incubated with 40 μg beads following the same isolation protocol timings and volumes

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Protocol MojoSort Mouse CD326 EpCAM Positive
Protocol MojoSort Mouse CD326 EpCAM Positive

Application notes To use this product in magnetic separation columns a titration of the Nanobeads should be performed Optimal concentration for magnetic separation columns is lotspecific Please contact BioLegend Technical Service tech for further assistance on how to use MojoSort Nanobeads in magnetic separation columns

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CD326 EpCAM MicroBeads mouse
CD326 EpCAM MicroBeads mouse

Proceed to magnetic separation 23 23 Magnetic separation Choose an appropriate MACS Column and MACS Separator according to the number of total cells and the number of CD326 EpCAM cells For details refer to the table in section 14

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Multifunctional Magnetic Particles for Combined
Multifunctional Magnetic Particles for Combined

Figure terization of magnetic particles a TEM image and b particle size distributions and PDI of magnetic particles after antiEpCAM modification c Magnetic hysteresis loop of magnetic particles at 300K with inset showing magnetic response of particles from a colloidal suspension using a permanent magnet

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PlusCellect Human EpCAM Kit
PlusCellect Human EpCAM Kit

Human EpCAM Selection Antibody Part 965766 625 L of biotinylated mouse antihuman EpCAM antibody Human EpCAM Detection Antibody Part 965767 250 L 25 tests of the magnetic separation step When processing greater than 2 x 10 8 cells use 17 x 100 mm 15 mL tubes

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MojoSort Mouse CD326 EpCAM Selection Kit CD326
MojoSort Mouse CD326 EpCAM Selection Kit CD326

Mouse CD326 EpCAM cells are selected by incubating the sample with biotin conjugated antimouse CD326 antibody after blocking with TruStain FcX antimouse CD1632 antibody then followed by Streptavidin Nanobeads The magnetically labeled fraction is retained by the use of a magnetic

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MojoSort Magnetic Cell Separation BioLegend
MojoSort Magnetic Cell Separation BioLegend

MojoSort is BioLegends magnetic cell separation system for the isolation and purification of cells from heterogeneous populations MojoSort offers outstanding performance at an excellent price Add some mojo to your experiments today Learn more about BioLegend’s cell separation system below

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Circulating Tumor Cells
Circulating Tumor Cells

Oct 22 2020 · CTCs enrichment is conducted with magnetic separation of EpCAMpositive cells whereas the detection is carried out by RTPCR for the identification of tumorassociated transcripts A clinical study conducted on patients with metastatic breast cancer used RTPCR for the analysis of the following transcripts GA7332 MUC1 and HER2

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Abstract 3963 Capture of EpCAMnegative mesothelioma
Abstract 3963 Capture of EpCAMnegative mesothelioma

Proceedings AACR 107th Annual Meeting 2016 April 1620 2016 New Orleans LA Backgrounds Circulating tumor cells CTCs as a surrogate of distant metastasis can be potentially useful in early diagnosis and monitoring therapeutic effects for patients with malignant tumors Among a variety of systems for detection of CTCs an epithelial cell adhesion molecule EpCAMbased immunomagnetic

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Epcam Miltenyi Biotec Bioz
Epcam Miltenyi Biotec Bioz

Western blotting For Western blot analysis Caki1 were separated from coculture with HUVEC by magnet bead separation with human CD326 MicroBeads EpCam epithelial cell adhesion molecule 100 μl Miltenyi Biotec Bergisch Gladbach Germany using a MidiMACS Separator Miltenyi Biotec Bergisch Gladbach Germany FACscan analysis

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Bioinspired DNA Nanointerface with Anisotropic Aptamers
Bioinspired DNA Nanointerface with Anisotropic Aptamers

MCF‐7 cells were incubated with 200 n m biotin‐labeled TDN‐3 EpCAM or DNA nanosynapse in BB buffer at 4 °C for 30 min with a final volume of 200 µL 5 µL Dynabeads of 10 mg mL −1 65 001 Thermo Fisher Scientific were then added and continued incubation for another 30 min followed by magnetic separation After that cells were

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Multifunctional Magnetic Particles for Combined
Multifunctional Magnetic Particles for Combined

Figure terization of magnetic particles a TEM image and b particle size distributions and PDI of magnetic particles after antiEpCAM modification c Magnetic hysteresis loop of magnetic particles at 300K with inset showing magnetic response of particles from a colloidal suspension using a permanent magnet

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Enrichment of circulating melanoma cells CMCs using
Enrichment of circulating melanoma cells CMCs using

as Epithelial Cell Adhesion Molecule or EpCAM Immunomagnetic antibodies against EpCAM have been used to target the CTCs followed by magnetic separation and optical analysis to isolate and reliably detect CTCs CellSearch Circulating Tumor Cell Test Janssen Diagnostics LLC Raritan NJ 3 10 This method of

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MojoSort Mouse CD326 EpCAM Selection Kit CD326
MojoSort Mouse CD326 EpCAM Selection Kit CD326

Mouse CD326 EpCAM cells are selected by incubating the sample with biotin conjugated antimouse CD326 antibody after blocking with TruStain FcX antimouse CD1632 antibody then followed by Streptavidin Nanobeads The magnetically labeled fraction is retained by the use of a magnetic

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A relevant immunomagnetic assay to detect and characterize
A relevant immunomagnetic assay to detect and characterize

METHODS The authors developed a single‐step assay that does not require density‐gradient separation This assay can be performed directly on crude human bone marrow aspirates and is based on the use of immunomagnetic beads coated with an antibody that recognizes an epithelial cell‐surface epitope the epithelial cell adhesion molecule EpCAM

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Magnetic Nanowire Networks for DualIsolation and
Magnetic Nanowire Networks for DualIsolation and

After magnetic separation the cfDNANW complexes were incubated in 500 µL of proteinase Kcontaining lysis buffer consisting of 128 M sucrose 40 mM TrisHCl 20 mM MgCl 2 4 Triton X100 and 50 mM DTT for 30 min at 60°C The cfDNANW complexes were washed twice with 1× TE buffer collected by magnetic separation and incubated in 20 µL

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Novel methods for the isolation of tumor cells from
Novel methods for the isolation of tumor cells from

Magnetic isolation of negative fraction ie tumor cells Magnetic labeling of nontumor cells Tumor cells transfected with eGFP CD326 EpCAMPE human Cultivation of tumor cells from primary specimens is frequently hampered by the presence of fibroblasts red blood

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An integrated enrichment system to facilitate isolation
An integrated enrichment system to facilitate isolation

Dec 14 2018 · Following magnetic separation the sample flows into the AF chip The AF chip is composed of glass with a wet etched channel 375 μm wide × 125 μm deep × 70 mm long Micronit Netherlands and an attached 215 MHz lead zirconate titanate piezoelectric PZT transducer Ferroperm Denmark

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Engineering magnetic nanoparticles and their integration
Engineering magnetic nanoparticles and their integration

Mar 22 2020 · Magnetic nanoparticles illustrated in Fig 2a were prepared in order to test their ability to enhance microfluidic device capture efficiency and specificity in cell mixtures under strong magnetic field gradients The nanoparticles were synthesized via thermal decomposition coated with polyethylene glycol and further modified with streptavidin to which biotinylated AntiEpCAM antibody

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Synchronous Detection of Circulating Tumor Cells in Blood
Synchronous Detection of Circulating Tumor Cells in Blood

Magnetic separation was repeated twice to further enrich for EPCAMexpressing cells A nucleic acid dye ThioflavinT BD Biosciences and a mAb to the leukocytespecific marker CD45 2D1 conjugated to peridininchlorophyllproteinCy55 were added to the sample

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ExosomeHuman EpCAM Flow Detection Reagent from cell
ExosomeHuman EpCAM Flow Detection Reagent from cell

ExosomeHuman EpCAM Flow Detection Reagent from cell culture enables detection of EpCAMpositive extracellular vesicles ECVs such as exosomes in enriched cell culture media using flow cytometry or electron microscopy Free exosomes alone are

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NanotechnologyBased Strategies for Early Cancer Diagnosis
NanotechnologyBased Strategies for Early Cancer Diagnosis

The cells captured on antiEpCAMcoated magnetic particles MPs were simply and efficiently trapped in the HB structured microfluidic device by an external magnetic field and then released from the device after removal of the magnetic field Aptamerconjugated upconversion nanoprobes assisted by magnetic separation for effective isolation

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